FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n02_A1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n02_A1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28046648
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC47650.012.17956911
TAGAACA60650.09.82853112
TCAGGTA65200.09.731087
GAATCGA46750.09.7290264
GGTAGAA59200.09.65127810
CGCGATA19050.09.44784629
CAGGTAG61700.09.4060848
GGTAAGT30100.09.34194317
AAAATCC103250.08.8986153
CGATAGT20800.08.8692831
TAATCGA34100.08.8481014
CTCGAAT32200.08.8120841
ACTTTCG20700.08.80535819
GGAAAAT128700.08.7839361
AATCGAA63650.08.771035
TTCCGAC32150.08.67906723
TAACGAT32700.08.66833844
ACATGCG48250.08.6272556
TGCGAGT44500.08.44170213
CGAAATT43800.08.22642
Produced by FastQC (version 0.11.5)