FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n02_A1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n02_A1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26601964
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC39800.010.2298411
CGTGACT23150.09.8247692
GTACTCG45750.09.7446734
TACTCGA49350.08.8950145
GAATTGA72150.08.8004714
CTCGAAT27600.08.4038861
GGAAAAT108100.08.3118781
TAGAACA49800.08.31116312
TCAGGTA53550.08.1509287
GAATCGA43350.08.1026794
AGTACTC58950.07.9454633
GATCTAG41750.07.9261095
GGGGAAT52150.07.8590321
GGTAGAA52800.07.71107110
AATTGAC48250.07.69813975
CTATAAG27850.07.59968854
AATCGAA52850.07.58183245
ATCTAGC46350.07.57323176
CACTCGT46400.07.563606335
AAAATCC79400.07.4872643
Produced by FastQC (version 0.11.5)