FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n02_A1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n02_A1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24596885
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC34200.012.82573611
CTCGAAT23900.010.9305911
GAATCGA42650.010.5590034
AATCGAA54700.010.1242845
GTACTCG44250.010.0754374
AAAATCC74250.09.8875513
TAGAACA46050.09.76954112
CGTGACT21800.09.7107982
ACGGTAC15100.09.68647320
TACTCGA46700.09.4001395
GGTAGAA46300.09.37666410
GGAAAAT98750.09.3504021
AGTACTC53150.09.10967353
GCGTGAC26750.09.0083151
TCAGGTA50950.08.8761137
CACGGTA16700.08.62368219
CGGCTAT33000.08.5306011
AAATCCT78000.08.2273924
AAAAATC72150.08.1780262
TCCGTTA32600.08.14504819