FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n02_A1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n02_A1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26577561
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG53350.010.930914
TACTCGA54900.010.7430075
GTAGAAC31000.010.3764811
AGTACTC64150.09.5830283
CGTGACT23300.09.3749142
CTCGAAT26350.09.1443871
AATTCAC39300.08.8211485
CACTCGT48850.08.79618535
GCGGAAA62750.08.6128921
GGAAAAT116250.08.6008531
AAAATCC86500.08.5387543
AATCGAA59100.08.3416815
ACTCGTG53400.08.00369436
TCACTCG52400.07.942660334
GAATCGA45300.07.90296174
CTCTATA53250.07.857931645
GAATTGA72300.07.84789564
AAATCCT87450.07.82716944
GCGTGAC31150.07.6630081
CGCGATA11750.07.65884329
Produced by FastQC (version 0.11.5)