FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n02_A1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n02_A1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29351517
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG56750.011.6650244
TACTCGA58400.011.3714255
CGTGACT27250.010.5782622
AGTACTC66800.010.4169423
GAATCGA45550.09.837144
GGAAAAT126550.09.6807021
TATAAGG32000.09.49706655
AATCGAA64800.09.3798195
GTAGAAC35600.09.162147511
CTATAAG33250.09.0743884
AAAATCC96900.08.8776463
CTCGAAT29550.08.7641641
GCGTGAC37300.08.7544571
CTCTATA58500.08.49850145
CACTCGT58950.08.472033535
ACTCGTG60700.08.11576636
AAATCCT97750.07.92402554
TCACTCG62300.07.87204734
GTGACTC39000.07.79520273
GAATTGA79350.07.7751334
Produced by FastQC (version 0.11.5)