FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n02_A1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n02_A1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26136948
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23500.011.5953352
GTACTCG55850.011.0063614
TACTCGA57500.010.5314675
GTAGAAC33400.09.9002511
AGTACTC68200.09.4765453
GCGTGAC32250.09.4269211
GGAAAAT115550.09.4133241
AAAATCC87350.09.3325043
GAATTGA71750.08.5987024
GAATCGA44500.08.2982814
CGTGTAG16750.08.19191416
AAATCCT87850.07.99681574
GCATAGG32400.07.98589741
TATAAGG28850.07.9590065
GTGACTC35950.07.95531373
AATCGAA57750.07.9521155
CGTTAAC23900.07.908842621
TCCGTTA23850.07.736722519
CTATAAG30200.07.6795284
TTACGAC26450.07.655119429
Produced by FastQC (version 0.11.5)