FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n02_A1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n02_A1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26675241
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25700.011.0408752
GTACTCG49300.010.0936044
CTCGAAT26250.09.5226931
AATCGAA56100.09.470055
GTAGAAC35300.09.36750811
GCGTGAC33450.09.3580371
TACTCGA52800.09.2944935
GAATCGA44050.09.200844
GAATTGA77250.08.9191574
GGAAAAT123800.08.8769811
AGTACTC60000.08.8200053
AAAATCC90900.08.81938653
AATTGAC49650.08.4332865
GATCTAG40900.08.2560265
AAAAATC83900.07.97187762
ACGGTAC13050.07.93183520
CACGGTA15800.07.690635719
GTGACTC38550.07.65242623
CGAATCG41800.07.650053
AAATCCT91950.07.6401834
Produced by FastQC (version 0.11.5)