FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n02_A1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n02_A1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26150237
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24050.013.0157342
GTAGAAC32150.011.33464111
GCGTGAC31400.010.9731151
GTACTCG59250.010.6407884
TACTCGA58650.010.5554865
CTCGAAT25750.010.1449261
ACGGTAC15100.09.98462820
AGTACTC67900.09.7506423
GGAAAAT124350.09.2361831
GAATCGA40950.09.0176654
CACGGTA16600.08.94684419
AAAATCC95650.08.9229793
AATCGAA53850.08.8208475
GAATTGA68350.08.7299444
TAGAACA42350.08.604691512
GTGACTC38300.08.4080053
AATTGAC46800.08.1774255
AAATCCT97150.08.1352034
GAAAATC106300.07.9021362
TCAGGTA51000.07.8524317
Produced by FastQC (version 0.11.5)