FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13696196
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA4450.032.1342416
CACACGT4500.029.83366812
ACGTCTG4700.029.4435215
CACGTCT5350.028.04905314
CTATTCG754.4014087E-827.70331213
ACACGTC5200.026.63779613
AGCACAC6150.023.64863810
CGACTAG550.003393473320.98735813
CAGTCAC5000.020.61981627
CGTTCTA1703.6379788E-1220.37008313
CCAGTCA5250.019.63792226
TCGGAAG9350.019.607813
ATCGGAA9500.019.298072
GATCGGA9800.018.9384251
CGGTTAG1353.189598E-818.8108913
AACTCCA7250.018.54856122
GTCGATA2500.018.45127516
CGTAACG759.409272E-418.45120418
CGTTAGC1658.8766683E-1018.17163818
CCGAGAC3600.018.05657429
Produced by FastQC (version 0.11.5)