FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n01_A1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n01_A1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32416010
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG443580.13683978996798185TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG75400.031.58318515
CCGTACG81350.031.45835733
CGTCTGA78600.030.20902816
CTCGTAT81600.030.12344444
TCGTATG84800.029.67236545
CGTAATC83150.029.3733138
ACCGTAC97350.026.770232
TACGTAA95150.026.63303836
ATCGGAA99050.026.3948062
TCGGAAG102850.025.6371923
ACGTAAT99050.025.38117237
CACGTCT96100.025.11717414
CACCGTA102100.025.04234531
CACACGT106700.024.62503212
GTAATCT101150.024.34598239
ACACGTC109100.022.16650613
TAATCTC114050.021.8470640
AATCTCG115650.021.64156241
CAGTCAC125750.021.02443327
AGTCACC128500.020.87274728
Produced by FastQC (version 0.11.5)