FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n01_A1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n01_A1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28046648
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG43950.032.09198833
ACGTCTG46450.029.3058915
AGTCACG49000.029.0570328
CGTCTGA49850.027.1213316
ACGTTTC55700.025.56220432
TTCGGAA56700.025.0303536
CTCGTAT53550.024.99783944
TTTCGGA57700.024.6746735
TCGTATG55650.024.17463745
TCGGAAG63300.023.3722443
ATCGGAA62650.023.3646952
GTTTCGG61900.023.18268634
GTCACGT62900.022.52882629
CACACGT68800.021.27370812
TCGGAAT69400.020.41775937
CACGTTT73950.019.49593431
CACGTCT72050.019.15033314
ACACGTC76400.018.30214913
CAGTCAC94850.016.26088327
GATCGGA92600.016.0512081
Produced by FastQC (version 0.11.5)