FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n01_A1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n01_A1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26601964
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG308300.11589369867578199TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG57100.029.74690415
AGTCACG63150.029.10695828
TCGTATG62150.028.9237745
CGTCTGA59550.028.60078216
CTCGTAT63000.028.53449444
CACGTGG68050.026.97894731
GTCACGT70500.025.94542729
GGCCTTA74700.024.57425136
GCCTTAT74650.024.38120337
TCGGAAG77550.024.1882463
ATCGGAA77900.023.9933642
CACGTCT72600.023.74669514
TATCTCG76200.023.6502741
CACACGT83700.022.59520112
TCACGTG81150.022.48550630
ACGTGGC83750.022.26893632
ACACGTC83400.021.05987713
CCTTATC89600.020.38816538
CTTATCT91950.019.81888839
CAGTCAC99400.019.21087827
Produced by FastQC (version 0.11.5)