FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n01_A1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n01_A1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24596885
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG41750.025.09313445
AGTCACG41950.024.70874828
ACGTCTG38700.024.7030315
CGTCTGA39700.024.13909316
CTCGTAT40000.022.8897144
CGTGAAA46450.022.26848233
ACGTGAA47100.021.91292232
CACGTGA49650.021.23840331
TGAAACG50800.020.1824335
ATCGGAA53300.019.8631532
AACGATC45600.019.78422238
GTCACGT52750.019.77745829
TCGGAAG55200.019.3010653
CACGTCT50450.019.17893214
CACACGT58950.018.56444412
AAACGAT55700.018.4071837
TCACGTG60650.017.4599730
GAAACGA59600.017.31515336
ACACGTC63700.015.77088113
CAGTCAC74050.015.02408427
Produced by FastQC (version 0.11.5)