FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n01_A1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n01_A1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26577561
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG49400.026.69567728
ACGTCTG45950.026.23658815
CTCGTAT52400.025.63379344
CGTCTGA48350.025.1256916
TCGTATG53950.024.89685645
ACGTCCG55650.023.77855932
TCGGAAG62800.021.6351053
GTCCGCA62000.021.59535834
ATCGGAA63050.021.5848242
GTCACGT61850.021.57545529
CCGCACA61650.021.35237336
CACGTCT62600.019.88652414
CACACGT69200.019.72872412
TCACGTC73700.018.04584730
CGCACAT73900.018.025137
ACACGTC72450.017.27848213
CAGTCAC83450.016.71325927
TCCGCAC80750.016.6901435
GATCGGA91950.014.9483761
CGGAAGA93400.014.90642454
Produced by FastQC (version 0.11.5)