FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n01_A1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n01_A1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29351517
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG53100.029.84003415
CTCGTAT59100.028.5256844
CGTCTGA56150.028.21916216
TCGTATG64400.026.31632245
GTCCGCA70800.023.96943736
ATCGGAA75650.023.3767322
TCGGAAG76800.023.2598723
CACGTCT70950.022.95191814
CACACGT78850.022.8793812
CGCATCT83950.020.02901539
CCGTCCG93050.019.63547934
ACACGTC88350.018.79801613
CAGTCAC97100.018.6298627
ACCCGTC94300.018.61586432
CGTCCGC101500.017.99914635
TCCGCAT95500.017.93429837
TCACCCG97550.017.78888930
AGTCACC103800.017.64487328
CCCGTCC102900.017.14755833
TCTCGTA101250.016.82744443
Produced by FastQC (version 0.11.5)