FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n01_A1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n01_A1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26136948
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG341540.1306732522863802TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG64250.032.080715
TCGTATG60850.030.7888545
CGTCTGA67150.030.66074616
CTCGTAT64350.028.87158444
ATCGGAA85650.026.4957392
TCGGAAG86050.026.3725223
CACACGT87100.026.20551112
CACGTCT81850.025.49345814
GTCAGAA91250.024.26020836
GTCACAT95450.023.42936529
TGTCAGA96900.023.16927335
AGTCACA98650.022.80534428
ACACGTC96050.021.9653713
CAGTCAC108100.021.24472427
AATCTCG99000.020.71101241
AGCACAC119200.019.84309210
GATCGGA116150.019.6562771
CGGAAGA116850.019.612634
CCAGTCA115000.019.56159626
TCACATG115500.019.4399430
Produced by FastQC (version 0.11.5)