FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n01_A1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n01_A1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26675241
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG396140.14850475015389739TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAT72900.033.5971237
AGTTCCG74200.033.37318834
CGTATCT74100.032.90283239
CCGTATC75000.032.5371838
ACGTCTG71500.032.3904315
CGTCTGA72800.031.71668816
TCGTATG78400.030.89651945
CTCGTAT78350.030.88841844
TTCCGTA84350.029.16883736
ATCGGAA92900.027.6862722
TCGGAAG93350.027.5527533
CACGTCT85850.027.38059814
GTTCCGT91750.026.96326635
CACACGT96900.026.84033412
TATCTCG94500.025.8465641
GTCACAG100850.024.7760629
ACACGTC99600.023.7631113
TCACAGT108050.022.85588330
AGTCACA111500.022.67039128
ACAGTTC111250.022.4603832
Produced by FastQC (version 0.11.5)