FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l03_n01_A1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l03_n01_A1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26150237
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG49100.029.70526115
CGTCTGA51150.028.24315516
TCGTATG55900.027.51008245
CTCGTAT52750.026.9046344
ATCGGAA68450.023.1173532
TCGGAAG70300.022.6045133
CACACGT71500.022.25380112
CACGTCT66550.021.98611514
CACAGTC74500.020.67512131
GTCACAG76300.020.50966529
AGTCACA82100.019.196828
TCACAGT81350.019.18158530
CAGTCAC90700.018.18903527
ACACGTC82600.018.16244913
AATCTCG80700.018.08572841
AGTCAAC88450.017.92037834
GATCGGA90100.017.7134931
ACAGTCA96150.016.85796532
AGCACAC97050.016.8563110
GTCAACA93650.016.80419735
Produced by FastQC (version 0.11.5)