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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-04-03, 17:04 based on data in: /beegfs/mk5636/logs/html/CBKDUACXX/2

        General Statistics

        Showing 20/20 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        CBKDUACXX_l02_n01_N1W1
        72.9%
        52%
        30.4
        CBKDUACXX_l02_n01_N1W2
        72.4%
        52%
        29.5
        CBKDUACXX_l02_n01_N1W3
        70.4%
        51%
        29.1
        CBKDUACXX_l02_n01_N1W4
        71.5%
        52%
        29.2
        CBKDUACXX_l02_n01_N1W5
        70.7%
        52%
        29.7
        CBKDUACXX_l02_n01_N1W6
        70.2%
        52%
        26.5
        CBKDUACXX_l02_n01_N1W7
        68.7%
        51%
        28.4
        CBKDUACXX_l02_n01_N1W8
        75.8%
        52%
        32.1
        CBKDUACXX_l02_n01_N1W9
        71.1%
        52%
        26.4
        CBKDUACXX_l02_n01_undetermined
        93.5%
        45%
        13.4
        CBKDUACXX_l02_n02_N1W1
        74.4%
        54%
        30.4
        CBKDUACXX_l02_n02_N1W2
        74.2%
        54%
        29.5
        CBKDUACXX_l02_n02_N1W3
        72.0%
        54%
        29.1
        CBKDUACXX_l02_n02_N1W4
        73.0%
        54%
        29.2
        CBKDUACXX_l02_n02_N1W5
        72.4%
        54%
        29.7
        CBKDUACXX_l02_n02_N1W6
        72.4%
        54%
        26.5
        CBKDUACXX_l02_n02_N1W7
        70.4%
        53%
        28.4
        CBKDUACXX_l02_n02_N1W8
        76.7%
        54%
        32.1
        CBKDUACXX_l02_n02_N1W9
        72.9%
        54%
        26.4
        CBKDUACXX_l02_n02_undetermined
        92.4%
        45%
        13.4

        Lane 2 Demultiplexing Report

        Lane 2 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 10/10 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        13,406,979
        4.9
        N1W1
        30,397,868
        11.1
        N1W2
        29,461,209
        10.7
        N1W3
        29,134,375
        10.6
        N1W4
        29,154,520
        10.6
        N1W5
        29,685,677
        10.8
        N1W6
        26,521,343
        9.7
        N1W7
        28,410,021
        10.3
        N1W8
        32,093,574
        11.7
        N1W9
        26,393,824
        9.6

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        TAAGTT
        139994.0
        1.0
        TAACTT
        102147.0
        0.8
        TAAGGT
        99184.0
        0.7
        CAAGTT
        95129.0
        0.7
        TAACGT
        84389.0
        0.6
        CCCCCC
        79579.0
        0.6
        CAACTT
        75410.0
        0.6
        TAAATT
        70884.0
        0.5
        CAAGGT
        70066.0
        0.5
        TCAGTT
        68788.0
        0.5
        CCCCGC
        67400.0
        0.5
        CAACGT
        64711.0
        0.5
        TACCTT
        63708.0
        0.5
        CCAGTT
        63597.0
        0.5
        CCACCC
        62352.0
        0.5
        TACGTT
        59167.0
        0.4
        GTTCGG
        57617.0
        0.4
        CAAATT
        57143.0
        0.4
        TAAAGT
        56851.0
        0.4
        TCACTT
        56505.0
        0.4

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        2.0
        292,624,096
        274,659,390
        4.9
        5.2

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

        loading..

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (51bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        loading..

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        20 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        No samples found with any adapter contamination > 0.1%

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.