FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13406979
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT5900.025.1883852
GTAGATC1850.023.10802732
AACCGCT2150.021.97666731
TGTAGGG3750.020.39635317
TAGATCT2150.019.88365233
GTGTAGA2200.019.43160630
TCGGAAG9300.019.1272493
TCTCGGT2300.018.58564437
ATCGGAA9600.018.2949732
GTGTAGG4200.018.21157316
TCGTGTA4400.017.89506114
GAGTGTA2550.017.64462928
GTAGGGA4500.016.99968518
GATCGGA10250.016.915571
AGTGTAG2550.016.76439529
AGCGGTT5300.016.12967310
CGGAAGA11100.016.023984
TAGGGAA5100.015.44112119
TGTAGAT2850.014.99994831
AGCGTCG6600.013.29347410
Produced by FastQC (version 0.11.5)