FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n02_N1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n02_N1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26393824
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC36000.011.0607511
CGTGACT20900.010.7741162
GGAAAAT124350.09.99601651
CACTCGT42800.09.40973635
AATCGAA51350.09.3379375
AAAATCC88700.09.1898213
GTACTCG42300.09.0485124
ATCTAGC49100.08.9826676
TCACTCG46150.08.97045134
GAATTGA73000.08.9134044
CTCTATA46550.08.69868345
ACTCGTG47450.08.58155636
GCGTGAC30050.08.5426781
AAATCCT92200.08.4735844
GCGGAAA56350.08.4717981
CGCGATA13050.08.4480629
CTCGAAT26700.08.4337831
TACTCGA45700.08.3742235
AATTGAC45050.08.3451375
GGTAGAA49650.08.3370510
Produced by FastQC (version 0.11.5)