FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n02_N1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n02_N1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32093574
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC41650.013.01712911
GGTAAGT53050.012.46720217
ACTTTCG39150.012.29882219
CGAAATT67550.011.5332712
GTACTCG51250.011.377784
GTAAGTT59100.011.3068518
TTCCGAC62100.010.97828723
AGTCTCG54750.010.9370555
ACATGCG66350.010.8528046
GAACGCT46600.010.76719321
CACGAAA62550.010.75513633
TTAAGTC59550.010.6627462
TGCGAGT63950.010.65898613
CGTGACT20000.010.5827762
TAAGTCT58150.010.570953
TAACGAT65550.010.39897444
GCGTAAC64500.010.32457641
TACTCGA56750.010.2739345
CGTAACG65600.010.25433642
CGTGCGA66800.010.03582311
Produced by FastQC (version 0.11.5)