FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n02_N1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n02_N1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28410021
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC40300.010.82956611
CACTCGT41500.09.65019335
GTACTCG37900.08.6731494
TCACTCG45050.08.64003434
GGAAAAT111550.08.6194961
ACTCGTG46500.08.51497436
GGTAGAA52300.08.430810
GCGGAAA57350.08.2453571
TCAGGTA56650.08.1820777
CTCTATA49550.08.03577945
TAGAACA56000.07.873765512
AAAATCC82850.07.82731533
TACTCGA42200.07.68171365
GGCGAAT25200.07.5952481
CGATAGT14000.07.55345131
CAGGTAG58750.07.54371078
CGCGTAG18600.07.499893733
CGCGATA14450.07.47391629
GAATCGA43100.07.4700234
CTCGAAT30350.07.34514051