FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n02_N1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n02_N1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26521343
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC43450.012.37438511
GTACTCG34800.010.1572624
GGTAGAA52950.010.02678110
TCAGGTA57600.010.0003427
TAGAACA53500.09.92370112
CGTGACT17400.09.4468012
ATTAGGG31150.09.1023276
GGAAAAT121150.09.0700911
CAGGTAG60150.08.9764758
ACTTTCG23500.08.9041919
TACTCGA39000.08.7738165
GAATCGA41400.08.592374
AAAATCC90500.08.4593733
CTCGAAT26650.08.4492481
CACTCGT31350.08.32507835
TCCGTTA49750.08.14064419
GAATTGA73750.08.0898434
TTAGGGT32350.08.0682827
AGATCAG97700.07.95024974
CGTTAAC49850.07.943773321
Produced by FastQC (version 0.11.5)