FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n02_N1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n02_N1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29685677
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC40000.012.20438811
CGTGACT27650.09.9351552
CACTCGT50200.09.63603835
AAAATCC99750.09.2776693
GGAAAAT129700.09.2360761
GTACTCG49000.09.1436914
TAGAACA53500.09.12477512
CTCTATA54550.09.11394545
TCACTCG54850.08.77810834
ACTCGTG56900.08.54015436
GGTAGAA58200.08.50385610
TCAGGTA60750.08.3337177
TCCGTTA34700.08.29956319
GCGGAAA69750.08.199841
ATCTAGC56550.08.1177156
TACTCGA55350.08.0123475
AAAAATC88150.07.94416142
GATCTAG55550.07.94297365
AGTACTC62500.07.9259743
CAGGTAG65250.07.7921898
Produced by FastQC (version 0.11.5)