FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n02_N1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n02_N1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29154520
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCGT53700.010.89338635
GTACTCG48050.010.7767584
ACTCGTG56050.010.35552536
CTCTATA58800.010.10028845
TCACTCG57350.010.00392834
CGTGACT28200.09.900852
GTAGAAC39950.09.85454711
TACTCGA52350.09.71826655
GCGGAAA72450.09.3237571
ATCTAGC56050.08.5513536
GAATCGA44200.08.4555044
GGAAAAT126550.08.3982341
GCGTGAC38100.08.3921171
AGTACTC66850.08.2520173
AATCGAA62500.08.1760385
CTCGTGA76100.08.1598137
TCCGTTA31600.08.1170619
GAATTGA76700.08.0134774
CGTAGGT16850.08.01152435
GCTCTAT76300.07.87234944
Produced by FastQC (version 0.11.5)