FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n02_N1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n02_N1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29134375
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCGT51900.010.53435435
ACTCGTG55600.09.67049936
CTCTATA57950.09.58843445
TCACTCG55950.09.57076534
GTAGAAC37900.09.31917111
GTACTCG45300.09.0953514
CGTGACT21700.09.0276322
AATCGAA59700.08.7861865
GCGGAAA73150.08.64987851
GGAAAAT129350.08.3036681
CGTGTAG16150.08.07927216
GAATCGA45100.08.0374064
CTCGAAT30450.07.9125141
TACTCGA50600.07.8303465
TCAGGTA52750.07.80602077
AAAATCC93200.07.7553733
CGTTAAC21950.07.68779321
CTCGTGA74400.07.6204337
ATCTAGC52600.07.52954966
GGGAAAT72050.07.40683031
Produced by FastQC (version 0.11.5)