FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n02_N1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n02_N1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29461209
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG83450.012.77927439
GTAGAAC47350.012.06792211
CGTGACT22200.011.4615282
TCAGGTA58800.010.7529447
GGGGGCC111000.010.559134544
GTACTCG52200.010.265134
TTTCGGG36400.010.26035437
TTCGGGG78250.010.17828438
TAGAACA58300.09.72413112
GGTAGAA62050.09.49900810
GGGGGGC175450.09.48878143
GGGGGAA176000.09.4216621
TACTCGA56250.09.3648745
CACTCGT53550.09.28539235
GGGGCCG83250.09.1874245
AAAATCC104900.09.1657023
CAGGTAG66850.09.1201228
GCGTGAC31850.08.9786861
GGAAAAT145600.08.8770571
CTCTATA55500.08.87666845
Produced by FastQC (version 0.11.5)