FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n02_N1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n02_N1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30397868
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24750.012.4638812
GTAGAAC45050.011.93495411
GCGTGAC36350.09.663471
TCAGGTA60700.09.6379157
GGTAGAA58150.09.20756710
TAGAACA59850.09.02120912
GGAAAAT143900.08.8566311
GAATCGA45750.08.808894
CACTCGT49600.08.75471335
CTCGAAT30650.08.5219781
TCACTCG50550.08.50116534
CTCTATA51000.08.42486645
GATCTAG50500.08.3805295
CAGGTAG68000.08.3372788
GTACTCG51500.08.3062644
GAATTGA81450.08.2649354
AAAATCC105400.08.2248513
AATTCAC48250.08.0714915
TCCGTTA38200.08.01042219
AATCGAA64650.07.93910035
Produced by FastQC (version 0.11.5)