FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13406979
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG6400.038.2796715
CGTCTGA6450.036.9375316
CACACGT7000.034.6800612
CACGTCT7150.033.36762214
AGCACAC7900.030.16010910
ACACGTC8300.028.97699413
TCGGAAG10300.028.5533143
CAGTCAC5950.028.01021227
ATCGGAA10700.027.4944112
CCAGTCA6450.026.88509226
GATCGGA11350.025.7263951
GCACACG9800.025.0008411
CTCCAGT8800.024.81579624
CGGAAGA12000.024.6953494
AACTCCA10500.022.2979722
AGAGCAC12350.020.0206538
CCCTACA2050.018.68382341
AGTCACT1608.094503E-1018.29746428
GAACTCC13250.017.66718121
GTCCTAT1053.0802155E-517.16621843
Produced by FastQC (version 0.11.5)