FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n01_N1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n01_N1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26393824
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG46600.027.90462915
CGTAATC45400.026.4170738
CGTCTGA49600.026.30756216
CCGTACG48000.026.2519633
TCGTATG48500.025.10214245
CTCGTAT49150.024.31291244
ACCGTAC59250.021.72231932
ATCGGAA59950.021.6941222
TCGGAAG61400.021.2548123
TACGTAA61150.020.12732736
CACGTCT66650.019.9489114
CACCGTA64200.019.7320731
CACACGT67000.019.7097912
ACGTAAT64050.019.00538437
GTAATCT66150.018.26888739
CAGTCAC74650.017.69351827
ACACGTC77950.017.6625413
AGTCACC83150.015.91201128
AATCTCG78650.015.70855741
TAATCTC78500.015.68120940
Produced by FastQC (version 0.11.5)