FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n01_N1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n01_N1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32093574
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG38000.032.3308933
AGTCACG43550.028.26223228
ACGTCTG44550.028.17850515
CGTCTGA47950.026.18044516
ACGTTTC52200.023.83667432
TTCGGAA51700.023.45816236
CTCGTAT46850.023.153244
TTTCGGA53900.022.91810235
TCGTATG48300.022.643945
ATCGGAA58150.021.5915052
GTCACGT57150.021.5749929
TCGGAAG61700.020.239713
CACACGT64500.019.7062812
CACGTCT66200.019.54054314
TCGGAAT63100.019.46987237
CACGTTT68050.018.48312431
CAGTCAC75950.017.00560227
GTTTCGG79000.016.34852234
ACACGTC81550.016.0275613
CGCGTAT30300.014.7801191
Produced by FastQC (version 0.11.5)