FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n01_N1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n01_N1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28410021
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG630050.22177033941650376TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG91600.034.99875615
AGTCACG90050.034.5578428
CGTCTGA93250.034.2829716
TCGTATG89400.033.4283845
CTCGTAT92250.032.64015244
CACGTGG97000.032.15064631
GTCACGT102550.030.34472329
CACGTCT106600.030.28486314
ATCGGAA106050.030.2135732
TCGGAAG107050.029.8681743
TATCTCG104250.029.24988241
CACACGT110350.029.17337812
GCCTTAT106550.028.8046737
ACGTGGC110950.028.43275332
TCACGTG113050.027.62530930
GGCCTTA112950.027.61026636
ACACGTC125600.026.09692613
CTTATCT119200.025.77039539
CAGTCAC124850.025.24947427
CCTTATC123650.024.89496638
Produced by FastQC (version 0.11.5)