FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n01_N1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n01_N1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26521343
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG268500.10123921703361703TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG50200.029.09039128
ACGTCTG54100.028.06988115
TCGTATG52750.027.81503345
CGTCTGA55450.027.26476516
CTCGTAT50250.025.9753344
CGTGAAA57250.025.58639733
ACGTGAA61000.023.93887732
CACGTGA62200.023.65795131
GTCACGT62400.023.40161129
TGAAACG62850.023.26994535
AACGATC55500.022.90734338
TCGGAAG67850.022.6502023
CACACGT68300.022.46366112
ATCGGAA67900.022.302182
TCACGTG66850.022.04545230
CACGTCT71850.021.32305714
AAACGAT67250.021.01189837
GAAACGA71900.020.27857836
CAGTCAC79700.019.2546427
ACACGTC82300.019.24374813
Produced by FastQC (version 0.11.5)