FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n01_N1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n01_N1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29685677
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG458850.1545694915430091TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG74300.032.1569415
AGTCACG73700.031.47772628
CGTCTGA76500.031.23216416
TCGTATG77000.029.83916945
ACGTCCG78950.029.41203532
CTCGTAT79050.028.8382944
TCGGAAG87700.027.1698553
GTCCGCA85700.026.91166734
ATCGGAA88050.026.883252
GTCACGT87700.026.83650629
CCGCACA89950.025.79000336
CACGTCT94800.025.67754714
CACACGT94450.025.46218912
TCACGTC98350.023.70136830
ACACGTC111200.022.05164513
CAGTCAC108200.021.83583527
CGCACAT106150.021.55746837
TCCGCAC111400.021.22818635
GATCGGA121300.019.758621
TCTCGTA119000.019.28924243
Produced by FastQC (version 0.11.5)