FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n01_N1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n01_N1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29154520
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG378850.12994554532196037TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG63550.031.82578815
CGTCTGA66650.030.17674416
CTCGTAT66600.028.5188144
TCGTATG69750.027.3268145
TCGGAAG78550.025.83793
GTCCGCA76950.025.64359536
ATCGGAA79200.025.4555172
CACGTCT81950.025.22887414
CACACGT87300.023.47584512
CGCATCT86800.022.32273139
ACCCGTC93200.021.48661432
CAGTCAC95900.020.99999427
CCGTCCG99200.020.64034334
ACACGTC102400.020.58528113
TCACCCG99600.019.97029930
AGTCACC105550.019.4211828
TCCGCAT102000.019.34599537
CCCGTCC107950.018.73910333
TCTCGTA102950.018.5367143
GATCGGA110550.018.382511
Produced by FastQC (version 0.11.5)