FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n01_N1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n01_N1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29134375
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA58500.029.2658216
ACGTCTG59400.028.93602215
TCGTATG54000.025.37879445
CTCGTAT55400.024.04782544
ATCGGAA73650.023.5855392
TCGGAAG76600.022.7945963
CACGTCT77950.022.16528114
CACACGT85500.020.36527812
ACACGTC92550.019.20287113
CAGTCAC91750.019.15387527
GTCAGAA92300.018.55111336
GTCACAT94550.018.22898529
AGTCACA96550.018.0620828
TGTCAGA96950.017.59181635
AATCTCG91300.017.15539741
GATCGGA106100.016.5024131
CCAGTCA110150.015.790132526
CGGAAGA114750.015.4123494
TCACATG114050.015.36847230
TCTCGTA90250.015.2355443
Produced by FastQC (version 0.11.5)