FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n01_N1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n01_N1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29461209
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG1429890.4853466807828558TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG179300.039.22100445
CTCGTAT182150.038.65799744
TCCGTAT184450.038.6578737
CGTATCT184400.038.4180539
AGTTCCG189600.038.3543134
CCGTATC187050.037.8816838
ACGTCTG196600.037.76339315
CGTCTGA198700.037.26238316
TTCCGTA197800.036.36659636
TATCTCG201700.034.97767341
GTTCCGT207050.034.94814735
ATCGGAA215400.034.4724542
TCGGAAG218450.033.990753
CACGTCT219500.033.9455714
CACACGT220150.033.86492512
GTCACAG217650.033.20559729
CAGTCAC222050.032.64994427
TCTCGTA216050.032.498543
TCACAGT221350.032.4466930
ACAGTTC223650.032.2542432
Produced by FastQC (version 0.11.5)