FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l02_n01_N1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l02_n01_N1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30397868
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG324800.10684959879423123TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG59900.030.3848715
CGTCTGA62200.029.18897216
TCGTATG60700.028.50899745
CTCGTAT59950.026.72712544
ATCGGAA77000.023.640912
TCGGAAG78600.023.3027323
CACACGT84950.021.79493912
CACGTCT86200.021.66228914
CACAGTC83250.021.43308331
CAGTCAC93750.019.58507727
GTCACAG96850.018.72518329
TCACAGT95600.018.64033330
ACACGTC101750.018.57223313
AGTCACA99400.018.13240428
ACAGTCA107050.017.25644932
GATCGGA109250.017.035861
AATCTCG97000.016.79678341
AGTCAAC108800.016.68902834
GTCAACA111600.016.12928235
TCTCGTA99250.016.09864243
Produced by FastQC (version 0.11.5)