FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12845395
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT5250.024.0206992
GTAGATC2500.019.79825232
GTGTAGA2350.019.14724730
GTAGAAC1454.796675E-918.61730811
GAGTGTA2500.017.09682728
TAGATCT2800.016.87364233
TGTAGAT3250.016.61391831
TCTCGGT2800.016.06950837
AGTGTAG2700.015.83193629
CGGTTCG3600.014.99727412
AGCGGTT4500.014.99721610
TCGGAAG7850.014.9173723
ATCGGAA8300.014.3798622
GTAGGGA3650.014.17881919
TCGTGTA3500.014.14028715
GATCGGA8650.014.06213951
GATCTCG4100.013.71819535
CGGAAGA8700.013.7180244
CCGGAAT1000.007500195413.5152791
TAGGGAA4050.013.33402520
Produced by FastQC (version 0.11.5)