FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n02_P1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n02_P1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33022483
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTCG51750.011.47876119
GCCCTAT60050.010.30231810
ATAGGGG60750.09.9246634
GTACTCG46550.09.7231034
CTCGAAT31900.09.5323351
GATAGGG64050.09.41381733
CGTGACT23800.09.3671712
TGCCCTA69300.09.3163169
GTAGAAC37250.09.24019411
GGAAAAT125300.09.1680151
TGGTAGG65850.09.08705927
CGCGATA20650.08.93382329
ATTAGGG61950.08.6468416
GGATAGA95000.08.64511420
TAGATCA97700.08.49832323
TAGGATA72100.08.36267130
AGTCTCG31500.08.3626245
TACTCGA50450.08.345415
GAATCGA46750.08.3328394
GGATAGG73200.08.32929732
Produced by FastQC (version 0.11.5)