FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n02_P1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n02_P1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24532896
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC39000.011.01739911
TAATCGA32200.010.6993794
CTAATCG29600.010.3464953
GGAAAAT114900.09.0960351
GGTAGAA49500.08.72582110
GAATCGA38800.08.70527654
CGCGATA17000.08.60240629
TAGAACA51650.08.44970812
CAGGTAG53000.08.2766728
TCAGGTA54550.08.2092957
GGTAAGT24400.08.20538517
AAAATCC88800.08.1909753
ATTAGGG33100.08.0917216
ACTTTCG26500.08.06629219
CTCGAAT29600.08.0661881
CGAATCG24800.07.99055623
AATCGAA53800.07.9510315
CGATAGT17300.07.933031631
TAACGAT28100.07.845158644
AATCGAG60150.07.822815
Produced by FastQC (version 0.11.5)