FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n02_P1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n02_P1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31968805
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC40350.010.81610211
ACTTTCG33800.08.92045319
GTACTCG48050.08.7634554
CTCGAAT34700.08.5686761
CACTCGT51900.08.36601935
AAAAATC94450.07.98712352
GGAAAAT119850.07.8373151
GGTAGAA54150.07.81033910
GCCCTAT42000.07.659444310
TAGAACA56750.07.6507512
GCGATTT74400.07.6295141
GAAAAAT173750.07.62292771
CGTGACT20500.07.5795392
TACTCGA51700.07.5772995
CGGGAAT22000.07.5766691
TCACTCG56050.07.54590134
ACTCGTG55300.07.525559436
GATCTAG47250.07.48089555
TCAGGTA60800.07.47644047
TCCGTTA62150.07.421839719
Produced by FastQC (version 0.11.5)