FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n02_P1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n02_P1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32526988
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC46950.011.6435511
CGTGACT25550.011.6343462
CTCGAAT37700.09.8584011
GTACTCG50450.09.5072744
GGTAGAA58400.09.28359610
GAATCGA58650.09.0611124
GGAAAAT138850.08.9386031
AATCGAA66050.08.8629295
TAGAACA62650.08.7975112
GCGTGAC39200.08.7916271
TACTCGA56250.08.5257755
TCAGGTA67750.08.46999557
CACTCGT54100.08.27525135
ATCTAGC47250.08.2410156
ACGGTAC20600.08.19199320
AAAATCC104050.08.1378073
AGTACTC66250.07.81814243
AAAAATC95700.07.788882
GCGGAAA72800.07.7661591
CGGCTAT43100.07.7347871