FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n02_P1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n02_P1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35060001
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT36550.012.0141912
GTACTCG70150.011.7484254
TACTCGA74550.010.9626415
GTAGAAC47600.010.39741811
GCGTGAC48500.010.2174321
AGTACTC83800.010.1039643
CTCGAAT39750.09.6332441
AAAATCC118500.09.4636553
GGAAAAT150450.09.08776951
AATCGAA71750.08.9429155
GAATCGA57800.08.8824594
TATAAGG40400.08.6935955
GTGACTC56150.08.5022543
CTATAAG41600.08.44418054
AAAAATC96550.08.3031912
GAATTGA93500.08.2605464
AAATCCT126450.08.1203034
TAGAACA61600.08.070888512
ATCTAGC56650.08.0256116
GAAAAAT187000.07.8415191
Produced by FastQC (version 0.11.5)