FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n02_P1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n02_P1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29647582
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG57950.010.802124
CGTGACT27650.010.5059942
TACTCGA61300.010.2469685
GTAGAAC36950.010.16759111
CTCGAAT34050.09.52572251
AGTACTC71100.09.3115143
AAAATCC91200.09.2346263
AATCGAA66550.09.2017945
CACTCGT54750.08.91675935
GGAAAAT121050.08.5408371
GAATCGA48400.08.4672754
GAATTGA75750.08.3827044
GCGTGAC39500.08.3824851
GCATAGG44600.08.37295941
AAAAATC87850.08.2538522
ACTCGTG58850.08.2183936
ACGGTAC15650.08.195209520
TCACTCG60000.07.949064334
TAGAACA49550.07.899912
GCGGAAA73700.07.79336931
Produced by FastQC (version 0.11.5)