FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n02_P1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n02_P1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28277940
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT20500.012.5227412
GTACTCG49350.010.54044
GTAGAAC36550.010.15566911
TACTCGA51150.09.8157415
GCGTGAC32800.08.5839731
AGTACTC66250.08.5317743
CTCGAAT29000.08.3107071
CACTCGT56550.08.27492735
GGAAAAT117400.08.2307871
ACTCGTG58800.07.91932336
AATCGAA59750.07.8377115
AAAATCC84500.07.80840543
TCACTCG59650.07.769448834
TAGAACA47750.07.585154512
GGTAGAA51900.07.542073710
CGCGATA12650.07.469762329
GCGGAAA75250.07.4532391
TCCGTTA36050.07.427501719
TCAGGTA54000.07.3343597
AGAACGG154550.07.32909633
Produced by FastQC (version 0.11.5)