FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n02_P1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n02_P1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27690944
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24500.011.3977072
GTAGAAC35500.011.02622611
GGAAAAT115200.09.2097881
GAATCGA43950.08.7615214
GCGTGAC35300.08.4232651
GTACTCG48150.08.4181854
TCAGGTA52600.08.2999117
TAGAACA49550.08.12672112
AAAATCC86800.08.0686953
GGGAAAT65300.08.0375561
GATCTAG44900.08.0232875
AATCGAA57250.07.98360825
GAATTGA79300.07.95109844
TACTCGA54000.07.75528675
AATCGAC31400.07.7441335
GGTAGAA53000.07.640163410
CTCGAAT27150.07.633071
CAGGTAG56900.07.5907388
CCGGAAT26400.07.5086181
AATTGAG114500.07.39368635
Produced by FastQC (version 0.11.5)