FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n02_P1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n02_P1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27221949
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC36400.09.33230311
AATCGAA53100.09.3279695
GAATCGA42550.09.3141464
GTACTCG50450.09.2393014
CGTGACT23150.09.1439022
GGAAAAT121750.08.6585631
TACTCGA53050.08.5728435
CTCGAAT29800.08.541441
AAAATCC87500.08.31281953
CGGGAAT22700.08.1368611
AGTACTC65150.07.7080473
GAATTGA83150.07.66397524
GCGTGAC32700.07.6461731
AATTGAC50250.07.4823725
CGAAAAT28700.07.4560811
GATCTAG40600.07.43083525
TCAGGTA49200.07.36386357
AAATCCT94750.07.3198374
TAGAACA45800.07.26958412
GAAAATC100850.07.23475462
Produced by FastQC (version 0.11.5)