FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12845395
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA3950.034.714716
ACGTCTG4050.033.85728515
CACACGT4650.030.43765412
CACGTCT4850.028.74009514
ACACGTC5200.026.35421213
AGCACAC6250.022.64270410
TCGGAAG8100.020.2445133
CAGTCAC4900.020.23079727
ATCGGAA8350.019.9083392
GCACACG7000.019.57741711
GATCGGA8850.018.5336381
CCAGTCA5600.018.09917426
CGGAAGA9450.017.827854
CTCCAGT7850.016.34909624
GAGACCG2850.015.80998231
AACTCCA8900.014.92624422
AGACCGA3250.014.55575232
CCGAGAC2950.014.5097829
CCGATTG1000.0076366513.4784832
CGAGACC3251.8189894E-1213.17041630
Produced by FastQC (version 0.11.5)